DMEM (2X) high glucose, no glutamine
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basic medium to support the growth of many different mammalian cells. Cells cultured successfully in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. We offer a variety of DMEM modifications for a range of cell culture applications.
DMEM is unique from other media because it contains 4 times the concentration of amino acids and vitamins than Eagle's original minimal essential medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM does not contain proteins, lipids or growth factors. Therefore, DMEM requires supplementation, usually with 10% fetal bovine serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
DMEM is manufactured in a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a manufacturer of medical devices and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical DMEM product manufactured in our Scottish factory (41965- 039). This facility is registered with the FDA as a manufacturer of medical devices and is certified to ISO 13485.
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basic medium to support the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the support selection tool.